| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165327 |
| Chromosome: | chromosome 6 |
| Location: | 6246967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290900 | (1 of 1) PF06294//PF11971 - CH-like domain in sperm protein (CH_2) // CAMSAP CH domain (CAMSAP_CH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACGTGGCTGGTGGCTGCCGTGAGAGAC |
| Internal bar code: | TGCGCGCAGGAGGACCTCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 98.2163 |
| LEAP-Seq n confirming: | 6002 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTTCCATCCTTTCGCAG |
| Suggested primer 2: | GGAGATCATCGCAGAGAAGG |