Insertion junction: LMJ.RY0402.165333_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.165333
Chromosome:chromosome 17
Location:1524312
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g707250 MFT29 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACTACACCATCTGTGCAGCCCAGCCGTA
Internal bar code:TTGGTGAGGACAAGCTACTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:953
LEAP-Seq percent confirming:96.9778
LEAP-Seq n confirming:6514
LEAP-Seq n nonconfirming:203
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTTATCTTTCCCCACCA
Suggested primer 2:TAACACAGACCACACCGCAT