| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165337 |
| Chromosome: | chromosome 4 |
| Location: | 2384800 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221050 | (1 of 3) PTHR12136//PTHR12136:SF47 - STEROIDOGENIC ACUTE REGULATORY PROTEIN STAR // STEROIDOGENIC ACUTE REGULATORY-LIKE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCAAAACAAAGCGGGGGCCAGGTGGG |
| Internal bar code: | TTCGGTATCGCTGCTGCTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 92.922 |
| LEAP-Seq n confirming: | 512 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCTCCGCCAATAACGTC |
| Suggested primer 2: | GTGCGACTCCTCTGTTGTGA |