| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165390 |
| Chromosome: | chromosome 14 |
| Location: | 310611 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609900 | (1 of 2) PTHR23130//PTHR23130:SF61 - FAMILY NOT NAMED // DEFENSE PROTEIN L(2)34FC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTGCTTGCGCACACAACCCCGCACCCA |
| Internal bar code: | CGGACAAATGGACGAACAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 97.1496 |
| LEAP-Seq n confirming: | 409 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTGGTAGAAGGAGCAG |
| Suggested primer 2: | TCTCAAACCTTGTTGGTCCC |