| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165396 |
| Chromosome: | chromosome 6 |
| Location: | 7439696 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGTGGCAGGAGGCTGTCAGGAAGCAAG |
| Internal bar code: | CTCTGGAGCGTGGGGGTGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 99.7573 |
| LEAP-Seq n confirming: | 9866 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGAAACAGAGGGGTGT |
| Suggested primer 2: | GGACGTTGGGACATACTGCT |