| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165409 |
| Chromosome: | chromosome 2 |
| Location: | 5329750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107000 | (1 of 1) PTHR24056//PTHR24056:SF11 - CELL DIVISION PROTEIN KINASE // CYCLIN-DEPENDENT KINASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGTAGAGCAGCCCCACCTCGCAGTCCG |
| Internal bar code: | TGAGATGCCTTTTCATAGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 90.535 |
| LEAP-Seq n confirming: | 1540 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTACTTTGGTGTTGC |
| Suggested primer 2: | CATCTTCGGAATGGTCCTGT |