Insertion junction: LMJ.RY0402.165434_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.165434
Chromosome:chromosome 8
Location:4454496
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g382350 TPT16,TPT14,EZY14 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 5) PTHR11132//PTHR11132:SF101 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGTTTTGGTGGCGTGAGTACTGACTGA
Internal bar code:GCTTGATGCCGGGCGTTAATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1028
LEAP-Seq percent confirming:98.4463
LEAP-Seq n confirming:2091
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGGAGGACATCCTGTCGG
Suggested primer 2:ACATTGCTGACTTGCTGACG