Insertion junction: LMJ.RY0402.165437_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.165437
Chromosome:chromosome 17
Location:2175949
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g713025 DNJ33 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; DnaJ-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTGTAGGTGGTTTGGTGGGTCGCAGCG
Internal bar code:CACACTCGTCGTGGCCCGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1059
LEAP-Seq percent confirming:70.4762
LEAP-Seq n confirming:18352
LEAP-Seq n nonconfirming:7688
LEAP-Seq n unique pos:137

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCCAGTAACAGCAGCAA
Suggested primer 2:ACGCACCTTCAGGTCTCTGT