| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165444 |
| Chromosome: | chromosome 1 |
| Location: | 5635297 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040100 | (1 of 1) PF13902 - R3H-associated N-terminal domain (R3H-assoc) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCATTAATCCAGCGGCGGCGCCGCCGC |
| Internal bar code: | GACGTCCACTACGGCCTCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.6787 |
| LEAP-Seq n confirming: | 5585 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGTCCTGCTAGGCAGTT |
| Suggested primer 2: | AATGCAGTGCAAGACGAGTG |