Insertion junction: LMJ.RY0402.165459_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.165459
Chromosome:chromosome 7
Location:2937474
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAACAAGTGCGCGCACGCCCATTGCGAT
Internal bar code:GCTCAGCGACCGCTCGCACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:453
LEAP-Seq percent confirming:98.1591
LEAP-Seq n confirming:1653
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCATTTCGACTTGTTGAG
Suggested primer 2:AGGTGTCCAGCTGCATCTTT