| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165463 |
| Chromosome: | chromosome 17 |
| Location: | 3174537 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721850 | SSA16 | cilia-sensing, structure and/or assembly; (1 of 1) K08292 - elongation factor 2 kinase (EEF2K) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGCTACGCCTTCGATTCTTAATTACTC |
| Internal bar code: | CCGAGGATGGATCCTGATTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 99.5181 |
| LEAP-Seq n confirming: | 413 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAACCCGCACCTTAAACA |
| Suggested primer 2: | CAGTGGAGATGTGAGAGGCA |