| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165464 |
| Chromosome: | chromosome 13 |
| Location: | 1827577 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575200 | PPP42 | (1 of 2) PTHR11668//PTHR11668:SF252 - SERINE/THREONINE PROTEIN PHOSPHATASE // SERINE/THREONINE-PROTEIN PHOSPHATASE BSL2-RELATED; Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGCGCCCTCGACAGTATGCCGCCAACG |
| Internal bar code: | CGAATCGCAGCTGAAGCTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.8143 |
| LEAP-Seq n confirming: | 1075 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGAGATGGATACGAGCCG |
| Suggested primer 2: | GCTTATGCTGACTTGCACCA |