| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.165487 |
| Chromosome: | chromosome_8 |
| Location: | 3426375 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g374700 | FAP244 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAAGCGGTCTAGTCTCGTAGTCTACCTCCC |
| Internal bar code: | ACTGTGTACAAGATACGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 91.1399 |
| LEAP-Seq n confirming: | 9443 |
| LEAP-Seq n nonconfirming: | 918 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGCCAGCCATCATTAC |
| Suggested primer 2: | TTCAGTGCATCTTGTACCGC |