| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165492 |
| Chromosome: | chromosome 6 |
| Location: | 2881789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272100 | (1 of 1) K15463 - tRNA A64-2'-O-ribosylphosphate transferase [EC:2.4.2.-] (RIT1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTCGTCACCTCAGACACGCCCTGCCCC |
| Internal bar code: | GGGCCTTGATTTGTCCCATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCTGCTGTTGGTGATGC |
| Suggested primer 2: | ATCCTGCTCTGCGAGTTCAT |