| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165504 |
| Chromosome: | chromosome 17 |
| Location: | 4262410 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730600 | (1 of 6) 3.2.1.4 - Cellulase / Endoglucanase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCGTGTGAATCGCAACTGCTGGGCAC |
| Internal bar code: | GATAGTCGCCATGCAGATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.6535 |
| LEAP-Seq n confirming: | 8916 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTGAAAGTTTTGCTGG |
| Suggested primer 2: | GGATGTGTTGCCTCAAACCT |