| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.165506 |
| Chromosome: | chromosome 11 |
| Location: | 1476284 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467740 | (1 of 1) PTHR10108//PTHR10108:SF705 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTCAGGGTGCCGGCAGCGGGTGTTGGG |
| Internal bar code: | AGGCTAGTGTGCAGCTGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1208 |
| LEAP-Seq percent confirming: | 98.8403 |
| LEAP-Seq n confirming: | 34007 |
| LEAP-Seq n nonconfirming: | 399 |
| LEAP-Seq n unique pos: | 187 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCTCGCACCTACTCGAC |
| Suggested primer 2: | GGGTTTTCATCGACCTAGCA |