Insertion junction: LMJ.RY0402.165506_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.165506
Chromosome:chromosome 11
Location:1476284
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467740 (1 of 1) PTHR10108//PTHR10108:SF705 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCACCCCATCGCTGCTCCCTCCCGCCT
Internal bar code:CTGATATATACCGCGCCCACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1208
LEAP-Seq percent confirming:99.5793
LEAP-Seq n confirming:29589
LEAP-Seq n nonconfirming:125
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTCGCACCTACTCGAC
Suggested primer 2:GGGTTTTCATCGACCTAGCA