| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165520 |
| Chromosome: | chromosome 3 |
| Location: | 519317 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145267 | (1 of 1) 3.5.4.3 - Guanine deaminase / Guanine aminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCTCAAAGCGCTGCAGGTCGTCCTCGC |
| Internal bar code: | CCGCACTACGACTCCGGATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 97.9874 |
| LEAP-Seq n confirming: | 1558 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGACCGTGCCTCCACT |
| Suggested primer 2: | ATTAATCACGCTCACCCACC |