| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165527 |
| Chromosome: | chromosome 5 |
| Location: | 1639505 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAAGGGACGAGATACTGTATTCCTCTGC |
| Internal bar code: | GGCCGGTAGAGGATGAGATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 76.4861 |
| LEAP-Seq n confirming: | 2033 |
| LEAP-Seq n nonconfirming: | 625 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGCCGTGTGTTAGGAAT |
| Suggested primer 2: | CCAACACCAACACACACACA |