Insertion junction: LMJ.RY0402.165530_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.165530
Chromosome:chromosome 13
Location:4126062
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g591650 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATACGTCAACAGCGCCGCCCACAACAGT
Internal bar code:ACACACGGGTCGTCGCCCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:378
LEAP-Seq percent confirming:98.1151
LEAP-Seq n confirming:6923
LEAP-Seq n nonconfirming:133
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGGTAAGCCAGCGTTCT
Suggested primer 2:CCAACCTGCCTTACCACTGT