| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165545 |
| Chromosome: | chromosome 16 |
| Location: | 5300514 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682450 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACGCTCCAGTCGGCCGCGGGTGTCCT |
| Internal bar code: | GTGAACGCACATTGCTGACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 99.8486 |
| LEAP-Seq n confirming: | 1978 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTAAGCGGCCTAGAAAG |
| Suggested primer 2: | GGCTGCAGCATAGTTGTTGA |