| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165552 |
| Chromosome: | chromosome 17 |
| Location: | 5117250 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735021 | (1 of 6) PTHR10582 - TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGGGCGAACTCGCGCGGGCCAAAGGT |
| Internal bar code: | CGCGCATGGGTTTCAGGAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 96.2938 |
| LEAP-Seq n confirming: | 24475 |
| LEAP-Seq n nonconfirming: | 942 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGCTCCGTCACTACTCA |
| Suggested primer 2: | CGTTCACTTTGGGCTGGTAT |