Insertion junction: LMJ.RY0402.165554_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.165554
Chromosome:chromosome 10
Location:904720
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g424300 KIN16A,KIN12-2,KIN16-1 (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTAACACCATGGACCGGGTCTGGTCGT
Internal bar code:ATAAGAATTTGTTACGCCGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:478
LEAP-Seq percent confirming:99.7677
LEAP-Seq n confirming:2577
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTACAACTCCACCACGGA
Suggested primer 2:GTGTTCTCACGCGATTGCTA