| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165561 |
| Chromosome: | chromosome 12 |
| Location: | 6530724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538900 | (1 of 25) PTHR10516 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCTGCATCGCAGCATCCGGACGCGTCG |
| Internal bar code: | AAGCGCTGAGCGGCAATTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 88.5376 |
| LEAP-Seq n confirming: | 4032 |
| LEAP-Seq n nonconfirming: | 522 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACCGCCTTCCAAATTAC |
| Suggested primer 2: | ACGTGCCTCAACTAGCACCT |