| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165688 |
| Chromosome: | chromosome 16 |
| Location: | 413425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGCAGGAAGCTTAGGAAGATGAAATC |
| Internal bar code: | ATGGGCGCGTTCTAGGGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 99.7418 |
| LEAP-Seq n confirming: | 5794 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGAGTGAGCGTTGGAGT |
| Suggested primer 2: | CGCGATTAGCTTTTAGCCAG |