Insertion junction: LMJ.RY0402.165720_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.165720
Chromosome:chromosome 16
Location:1752112
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCCCTGTGAGGCCTACTTCGAGGGAGT
Internal bar code:GGGATCGACCCGCCGGTTCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:88.5714
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCGTCCAGTACGGCAATC
Suggested primer 2:CGACCTAGGAGATGGTTGGA