Insertion junction: LMJ.RY0402.165720_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.165720
Chromosome:chromosome 16
Location:1752215
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTGCGGCCGAAGCGGCTGACGGTGTCC
Internal bar code:TCGTCGCTAAGACACGAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:503
LEAP-Seq percent confirming:99.5413
LEAP-Seq n confirming:1736
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCGTCCAGTACGGCAATC
Suggested primer 2:CGACCTAGGAGATGGTTGGA