| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165722 |
| Chromosome: | chromosome_16 |
| Location: | 7719367 |
| Confidence (%): | 95 |
| Locus disrupted | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g691440 | FAP43 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACAACGTCACACATGTGACAACATTTAGC |
| Internal bar code: | ATGGAAAGGCCGAGCTGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.6633 |
| LEAP-Seq n confirming: | 1184 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCCGAATCACTCCATCC |
| Suggested primer 2: | AGGGCAGTAACATGACCAGG |