Insertion junction: LMJ.RY0402.165722_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.165722
Chromosome:chromosome_16
Location:7719367
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre16.g691440 FAP43 Flagellar Associated Protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CACAACGTCACACATGTGACAACATTTAGC
Internal bar code:ATGGAAAGGCCGAGCTGGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:696
LEAP-Seq percent confirming:99.6633
LEAP-Seq n confirming:1184
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGCCGAATCACTCCATCC
Suggested primer 2:AGGGCAGTAACATGACCAGG