| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165725 |
| Chromosome: | chromosome_3 |
| Location: | 2322680 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g158400 | FAP218 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGGTCAAGGAATGAAGATCAGGGATACC |
| Internal bar code: | GGGAAGTCTCCAATTAGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 86.0575 |
| LEAP-Seq n confirming: | 1827 |
| LEAP-Seq n nonconfirming: | 296 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATGACCGTGCTTGAGAG |
| Suggested primer 2: | GGCGCATTACATGAAAAGGT |