Insertion junction: LMJ.RY0402.165726_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.165726
Chromosome:chromosome 6
Location:4139809
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278226 (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAACCTGTTGATCAAGTGCGCTGCCCTC
Internal bar code:AGTCGGTCACATAATCGTGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:989
LEAP-Seq percent confirming:98.5428
LEAP-Seq n confirming:3990
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACACCGT
Suggested primer 2:ATCTCCTCATCCTCCTCCGT