| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165728 |
| Chromosome: | chromosome 12 |
| Location: | 2699204 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503800 | RMT5 | Putative protine N-methyltransferase; (1 of 2) 2.1.1.127//2.1.1.259 - [Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase / RuBisCO methyltransferase // [Fructose-bisphosphate aldolase]-lysine N-methyltransferase / (dimerizing)]-lysine 6-N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTTTGCGAGCATGTTCTACTCCTGGCT |
| Internal bar code: | GCGCGGGTTTATATAATTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 99.5925 |
| LEAP-Seq n confirming: | 1222 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATCATAATGGCCCCCAC |
| Suggested primer 2: | GTAGCAGTGGCGGTAGTGGT |