| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165728 |
| Chromosome: | chromosome 16 |
| Location: | 5481706 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680850 | OPR103,MDA1 | atpA mRNA stabilization factor; (1 of 19) PTHR21228//PTHR21228:SF20 - FAST LEU-RICH DOMAIN-CONTAINING // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGCAGGGTGATCCTGCAGGGACTGACG |
| Internal bar code: | CGTGCCCCCCAATATGCTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 90.1099 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTGGCTGAAAATCCTG |
| Suggested primer 2: | CTCCATGAGCTGGCTTCTGT |