| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165757 |
| Chromosome: | chromosome 17 |
| Location: | 6826603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745097 | FAS15,FLA10 | FAS1 domain containing protein;; (1 of 1) IPR016112 - Group II dsDNA virus coat/capsid protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGAGGACGCCAGCGAGCGTGTGGGGTC |
| Internal bar code: | TACCTGCCTATGCGGCACGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 5.37634 |
| LEAP-Seq n confirming: | 150 |
| LEAP-Seq n nonconfirming: | 2640 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTTGGTCGAGTTGAGC |
| Suggested primer 2: | CACCCCAGTGATTGACTCCT |