| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165762 |
| Chromosome: | chromosome 1 |
| Location: | 2456700 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013800 | SXD1,TCY1 | (1 of 1) PF14392 - Zinc knuckle (zf-CCHC_4); Zinc finger containing protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGAAGAACCCGCTGCAGGCTGGCAGAG |
| Internal bar code: | GCCCTTCAAAGTTCGGATCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 99.5621 |
| LEAP-Seq n confirming: | 2956 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGAGAGGTGAGCGTGT |
| Suggested primer 2: | CAGTACCACCCCTCGAAAAA |