| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165777 |
| Chromosome: | chromosome 14 |
| Location: | 1546541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618600 | MRP7 | ABC transporter, multidrug resistance associated protein | 3'UTR|3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGACGGCAGTGCAGGAGCGCGCGGCCG |
| Internal bar code: | GGTGCTTAGCAAATTCTGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.705 |
| LEAP-Seq n confirming: | 338 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAATACGAGGCTCACAA |
| Suggested primer 2: | AATCCACGCCCTGACTTATG |