Insertion junction: LMJ.RY0402.165778_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.165778
Chromosome:chromosome 5
Location:2554903
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g236039 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGGACCCAAAATGCCTAGTTTGTAGCCG
Internal bar code:TCTCGGAGCTGATTTCTGAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:200
LEAP-Seq percent confirming:99.5247
LEAP-Seq n confirming:1047
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCAACAAGTCCGAGATCA
Suggested primer 2:ATCGCTGTTTATCGCTTGCT