| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165792 |
| Chromosome: | chromosome 2 |
| Location: | 1683259 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085850 | ARS6 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCTCGCCCAAGAAGCGGCGCCGGCGC |
| Internal bar code: | TAGCCCGGGTATAAGGTTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 99.5396 |
| LEAP-Seq n confirming: | 6270 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGCAGTACACGCAATG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |