| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165812 |
| Chromosome: | chromosome 9 |
| Location: | 2091908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393850 | MAPKKK2,PTK28,MAPKKK2 | Mitogen-Activated Protein Kinase Kinase Kinase; (1 of 1) IPR000719//IPR001229//IPR001245//IPR002290//IPR011009//IPR020635 - Protein kinase domain // Jacalin-like lectin domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGGTGGGAGAGGAGCAATTGGGCAGC |
| Internal bar code: | GGCAGGTAATGCGACTGTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 99.7586 |
| LEAP-Seq n confirming: | 2066 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGCCAGCATCACTCTGT |
| Suggested primer 2: | AGGCCAGTCCCATGTAGTTG |