| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165815 |
| Chromosome: | chromosome 12 |
| Location: | 2644876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCGCTGCAAGGAAAAACTGCAGCCGCA |
| Internal bar code: | TGGAGGTAGGTCAGCGCACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 89.9002 |
| LEAP-Seq n confirming: | 1531 |
| LEAP-Seq n nonconfirming: | 172 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGCAACAAATGCATCC |
| Suggested primer 2: | CCCTGGAAACTGCGTGTATT |