Insertion junction: LMJ.RY0402.165819_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.165819
Chromosome:chromosome 9
Location:7349172
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g413100 (1 of 16) IPR005069//IPR029044 - Nucleotide-diphospho-sugar transferase // Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCACATCCTGCCACGCATTTGCTGTCTG
Internal bar code:AAGGAGGTACCGCTATATGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1131
LEAP-Seq percent confirming:95.6035
LEAP-Seq n confirming:4610
LEAP-Seq n nonconfirming:212
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCTGACGCTGGAACTTCT
Suggested primer 2:GACTTTGGGTATCAGGCCAA