Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.165826 |
Chromosome: | chromosome_3 |
Location: | 5492628 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g185650 | FAP251 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATAGCAGATTTTGTCCGTGTAGCCATCGGA |
Internal bar code: | CAGAAGGTAGCACCCGGTCCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1106 |
LEAP-Seq percent confirming: | 99.1961 |
LEAP-Seq n confirming: | 4319 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCAGCAACACCTGCTGTA |
Suggested primer 2: | TTCAAAGCGTAAGCGGCTAT |