| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165834 |
| Chromosome: | scaffold 19 |
| Location: | 142984 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750897 | (1 of 1) 3.5.4.12 - dCMP deaminase / Deoxycytidylate deaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTAACCCTTGACTGTTTCAGACTCATGT |
| Internal bar code: | GCGTAGGCTCGCGTTTCGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 99.139 |
| LEAP-Seq n confirming: | 806 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGGAAACCCCAATCAT |
| Suggested primer 2: | TTTCCATCTCCGGATGAGAC |