| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165850 |
| Chromosome: | chromosome 5 |
| Location: | 791490 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248250 | (1 of 1) 1.4.1.24 - 3-dehydroquinate synthase II / DHQ synthase II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGGGTGAGAGCAACCACCCCCTCTCAC |
| Internal bar code: | TACAGCGCTTCGCCTGTGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 97.8123 |
| LEAP-Seq n confirming: | 1386 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATAAGAGCGAGGAGGA |
| Suggested primer 2: | TTGGGTTCGGTCTAGTTTGG |