| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165855 |
| Chromosome: | chromosome 12 |
| Location: | 2101209 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509250 | EYE2 | Eyespot assembly protein 2; (1 of 1) PTHR18929//PTHR18929:SF99 - PROTEIN DISULFIDE ISOMERASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTCGCAACACTTCGTTAAGTCTTCTAG |
| Internal bar code: | TGGCGCGGCTGTTGCATGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.6507 |
| LEAP-Seq n confirming: | 3138 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCTTGTTGAGCTCCT |
| Suggested primer 2: | TTCCAATATTCTTCTGGGCG |