| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165858 |
| Chromosome: | chromosome 12 |
| Location: | 472697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493400 | SMC4 | (1 of 1) K06675 - structural maintenance of chromosome 4 (SMC4); Structural Maintenance of Chromosomes protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCCACCGCTCATGCGGCCGCCTGCAC |
| Internal bar code: | GGACGACCGTGGCCGCTTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGCAGCTTCTGTGTGT |
| Suggested primer 2: | AAGGAGCACTTCTTCGTGGA |