Insertion junction: LMJ.RY0402.165869_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.165869
Chromosome:chromosome 2
Location:2303390
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g090550 ARS9 Arylsulfatase; (1 of 1) PTHR10342//PTHR10342:SF228 - ARYLSULFATASE // EXTRACELLULAR SULFATASE SULF-1 HOMOLOG intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCAAGTCAGCAGTCTCTCCGTCGTGCAC
Internal bar code:CGGTGGTTCCTCGGGCTGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:220
LEAP-Seq percent confirming:99.811
LEAP-Seq n confirming:4224
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGGTCTACTCCAGGTACA
Suggested primer 2:GAGACACAACGCTCTGTGGA