Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.165898 |
Chromosome: | chromosome_2 |
Location: | 5087917 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g104900 | FAP204 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGGCCGGGAATTTTGCGGCGCACATGTG |
Internal bar code: | TATAGTGAACTTGGGGTTTGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 441 |
LEAP-Seq percent confirming: | 99.7072 |
LEAP-Seq n confirming: | 4427 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTACCGAGACATCGCTGA |
Suggested primer 2: | ATTCATCAACGCAACTTCCC |