| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165904 |
| Chromosome: | chromosome 10 |
| Location: | 162421 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418900 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCGCTTGGCGTCGTACGACGTTACGA |
| Internal bar code: | GGTGCTTTATATGCCAAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 99.4497 |
| LEAP-Seq n confirming: | 2711 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGTCCTGCTCAGGGAAG |
| Suggested primer 2: | TGCTCTGATGGTTGAGTTGC |