| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.165909 |
| Chromosome: | chromosome 9 |
| Location: | 2123639 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393600 | (1 of 1) K10735 - GINS complex subunit 4 (GINS4, SLD5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCCCTCCCCCACCGACGGGGCTACGCG |
| Internal bar code: | GGCCGTGGGGAGAGGAATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 89.1008 |
| LEAP-Seq n confirming: | 654 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGCACTACGAAATGAG |
| Suggested primer 2: | ACAGAGGGTGTACAGGGGTG |