Insertion junction: LMJ.RY0402.165913_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.165913
Chromosome:chromosome 1
Location:4261384
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g028600 SDR2 (1 of 1) IPR002347//IPR003560//IPR013968 - Glucose/ribitol dehydrogenase // 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACACCCTGCCAGTCGCAGGACTTTGTAA
Internal bar code:TAACAAATGGAGAGTCCCGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:186
LEAP-Seq percent confirming:99.2933
LEAP-Seq n confirming:281
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTTGTAGATACCAGCCCA
Suggested primer 2:CATGAATCTGGTGGTGATCG